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Användning av kapslar för negativ färgning av virala prov inom biokonstruktion
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
DOI:

08:22 min

July 19, 2017

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Chapters

  • 00:05Title
  • 01:08Experiment Preparation in a BSL-2 Environment Prior to Working with the Virus Samples (Biocontainment Procedures were Filmed in BSL-4 Training Lab)
  • 01:45The Capsule Method for Negative Staining in Biocontainment Using Aqueous Glutaraldehyde and 1% Osmium Tetroxide Vapor Inactivation
  • 04:24The Capsule Method for Inactivation in Biocontainment 2% Glutaraldehyde Followed by Negative Staining in BSL-2 Laboratory
  • 05:50Results: Analyzing the Capsule Method of Negative Staining Viral Samples
  • 07:14Conclusion

Summary

Automatic Translation

Detta protokoll ger instruktioner för negativa färgprov som enkelt kan användas i BSL-2, -3 eller -4-laboratorier. Det innefattar användningen av en innovativ processkapsel som skyddar transmissionselektronmikroskopi gallret och ger användaren enklare hantering i de mer turbulenta miljöerna inom biokonstruktion.

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