/

Immunology and Infection

/

人单核细胞趋化体外和小鼠淋巴细胞贩运体内

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Please note that all translations are automatically generated. Click here for the English version.

人单核细胞趋化体外和小鼠淋巴细胞贩运体内

DOI:

10.3791/56218-v

•

08:38 min

•

October 30, 2017

•

Eliza Prangley, Terrence Kumar, Manish P. Ponda

¹Laboratory of Biochemical Genetics and Metabolism,The Rockefeller University

Chapters

00:05Title
00:51In Vitro Chemotaxis
02:56Murine Lymphocyte Adoptive Transfer
05:17Collection and Analysis
06:28Results: Representative In Vitro and In Vivo Migration Analyses
07:44Conclusion

Summary

Automatic Translation

淋巴细胞趋化和迁移定量评估的协议是免疫学研究的重要工具。在这里, 一个体外协议的描述, 允许 real-time, 多路复用的细胞迁移评估, 以及一个互补的体内技术, 使本机细胞跟踪脾脏。

Tags

Monocyte Chemotaxis In Vitro In Vivo Lymphocyte Trafficking Immunology THP-1 Cells Fluorescent Labeling Cell Migration MCP-1 HEPES RPMI-1640 C57BL/6 Mouse Adoptive Transfer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

迁移人T淋巴细胞分离，培养和分析<em在体外</em

一个<em>在体外</em>共感染模型研究<em>恶性疟原虫</em人原发性单核细胞源性的免疫细胞-HIV-1相互作用

量<em在体外</em>激活的主人微血管内皮细胞在静态条件下测量的检测，以中性粒细胞粘附

从表型分析和人体皮肤淋巴细胞分离<em>离体</em>细胞培养

人体血液中测粒及单核细胞吞噬功能和呼吸爆发活动

小鼠淋巴细胞通过标签<sup> 64</sup>的Cu-抗体受体靶向用于<em>体内</em>由PET / CT细胞运输

使用的淋巴细胞外渗分析<em>体外</em>人类血脑屏障模型

小鼠与人肺泡巨噬细胞的分离和体外培养

体外流动下单核细胞亚群的同时研究

单核细胞化学策略活性在Vivo的定量和血液单核细胞衍生巨噬细胞的特征

Read Article