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Regeneración de microelectrodos de oro vestidas para un analizador en tiempo real de la célula
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1School of Public Health,Nanjing Medical University, 2School of Pharmacy,Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering,Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research,The Affiliated Cancer Hospital of Nanjing Medical University
Chapters
- 00:04Title
- 00:52Proliferation/Cytotoxicity Experiment and Data Acquisition
- 03:54Case 1: Regeneration of Electronic Plates after Cytotoxicity Evaluation of Anti-cancer Drug
- 05:53Case 2: Regeneration of Electronic Plates after Drug Delivery using Mesoporous Material
- 07:34Assessment of Regeneration Effect
- 10:22Results: Evaluationof Regenerated Electronic Plates
- 12:01Conclusion
Este protocolo describe una estrategia general para regenerar comerciales microelectrodos de oro vestidas para un analizador de célula libre de etiqueta para ahorro en los alta ejecución costos ofmicrochip-ensayos. El proceso de regeneración incluye la digestión de la tripsina, enjuague con agua y etanol y un paso de giro, que permite el uso repetido de los microchips.
