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Forbedret metode til etablering af et In Vitro blod - hjerne barrieren Model baseret på svin hjernen Endothelial Cells
JoVE Journal
Medicine
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JoVE Journal Medicine
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
DOI:

09:23 min

September 24, 2017

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Chapters

  • 00:05Title
  • 00:44Purification of Porcine Brain Capillaries
  • 03:14Cultivation of Primary pBECs
  • 06:53TEER Measurements
  • 07:47Results: Representative Time Course of Initial Cultures of pBECs and Rat Astrocytes
  • 08:43Conclusion

Summary

Automatic Translation

Formålet med protokollen er at præsentere en optimeret procedure for etableringen af et in vitro- blod - hjerne barrieren (BBB) model baseret på primær svin hjernen endothelial celler (pBECs). Modellen viser høj reproducerbarhed, høj tæthed, og er velegnet til studier af transport og intracellulære menneskehandel drug discovery.

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