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小鼠外周动脉疾病模型中单核细胞归巢和肿瘤相关血管生成的活体显微镜观察
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JoVE Journal Medicine
Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease
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小鼠外周动脉疾病模型中单核细胞归巢和肿瘤相关血管生成的活体显微镜观察

DOI:
10.3791/56290-v

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08:38 min

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August 26, 2017

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, , , , , , , ,
1Department of Cardiology and Angiology,University of Magdeburg, 2Leibniz Institute for Neurobiology, 3Institute of Molecular and Clinical Immunology,University of Magdeburg, 4Division of Hematology/Oncology,Boston Children’s Hospital, Harvard Medical School

Chapters

  • 00:00Title
  • 00:39Cell Preparation
  • 01:54Anesthesia
  • 02:00Implantation of Basement Membrane-like Matrix
  • 03:35Tail Vein Injection
  • 05:04Intravital Microscopy
  • 06:07Representative Results
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

单核细胞是外周动脉疾病中 arteriogenesis 的重要介质。本协议采用基底膜样基质和活体显微镜, 研究单核细胞注射在股动脉结扎小鼠模型中的单核细胞归巢和肿瘤相关血管生成。

Tags

Intravital MicroscopyMonocyte HomingTumor-related AngiogenesisPeripheral Arterial DiseaseCell LabelingMatrigelVEGFBFGFHeparinSubcutaneous InjectionSystemic Cell Application

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