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用细胞色素 P450s 检测亚硝胺的体外Nitrosamide 形成的一般方法
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Cancer Research
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JoVE Journal Cancer Research
A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
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用细胞色素 P450s 检测亚硝胺的体外Nitrosamide 形成的一般方法

DOI:
10.3791/56312-v

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07:38 min

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September 25, 2017

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, ,
1Department of Pharmacology,University of Minnesota, 2Masonic Cancer Center,University of Minnesota

Chapters

  • 00:05Title
  • 00:46Preparation of Nitrosamide Positive Control
  • 03:07Nitrosamine-P450 In Vitro Incubation
  • 04:58Results: Nitrosamide Standard Preparation and In Vitro Incubation
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

细胞色素 P450s 致癌亚硝胺的α-羟基化是公认的代谢途径, 产生 DNA 损伤的中间体, 导致突变。然而, 新的数据表明进一步的氧化 nitrosamides 可能发生。我们描述了一个一般的方法, 检测 nitrosamides 产生的体外细胞色素 P450-catalyzed 代谢的亚硝胺。

Tags

NitrosamideNitrosamineCytochrome P450In Vitro MetabolismMass SpectrometryCarcinogenesisNorcotinineAcetic AnhydrideSodium NitriteColumn ChromatographyNMRBaculosomesNADPH Regeneration System

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