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Cancer Research

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JoVE Journal Cancer Research
En allmän metod för att upptäcka Nitrosamide bildning i In Vitro metabolismen av nitrosaminer genom cytokrom P450s
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En allmän metod för att upptäcka Nitrosamide bildning i In Vitro metabolismen av nitrosaminer genom cytokrom P450s

Article DOI: 10.3791/56312-v • 07:38 min • September 25th, 2017
September 25th, 2017 •
1,2, 2, 2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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Α-hydroxylering av cancerogena nitrosaminer genom cytokrom P450s är den accepterade metaboliska smittvägen som producerar DNA-skadande intermediärer, som orsakar mutationer. Nya data tyder dock ytterligare oxidation till nitrosamides kan uppstå. Vi beskriver en allmän metod för att upptäcka nitrosamides produceras från in vitro- cytokrom P450-katalyseras metabolismen av nitrosaminer.

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Cellbiologi problemet 127 nitrosamin nitrosamide cytokrom P450-metabolism processive oxidation carcinogenes högupplösande masspektrometri
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