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JoVE Journal

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Immunostaining for DNA modifikasjoner

Immunostaining for DNA modifikasjoner: beregningsorientert analyse av AC Confocal bilder

Article doi: 10.3791/56318
September 7th, 2017

Summary September 7th, 2017

Please note that all translations are automatically generated.

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Oppdagede nylig oksidert former for 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) og 5-carboxylcytosine (5caC) kan representere distinkte DNA endringer med unike funksjonsroller. Her beskrives en semi kvantitative arbeidsflyt for visualisering av oxi-mCs' romlige fordelingen, signalet intensitet profilering og colocalization.

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