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Neuroscience
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粉扑诱导的全细胞电流在脑切片中的应用
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
Please note that all translations are automatically generated.
Click here for the English version.
粉扑诱导的全细胞电流在脑切片中的应用
DOI:
10.3791/56387-v
•
07:32 min
•
October 12, 2017
•
Yangjian Feng*
1,2
,
Binliang Tang*
1,2
,
Ming Chen
,
Li Yang
3,4,5
1
School of Psychology
,
South China Normal University
,
2
School of Life Sciences
,
South China Normal University
,
3
Brain Science Institute
,
South China Normal University
,
4
Center for Studies of Psychological Application
,
South China Normal University
,
5
Guangdong Key Laboratory of Mental Health and Cognitive Science
,
South China Normal University
Chapters
00:05
Title
00:54
Preparation of Prefrontal Cortex Slice
02:57
Whole-cell Patch Recording
05:57
Results: Dose and Puff-duration Response Curves
06:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述的粉扑技术, 其中的药理试剂可以在整个细胞膜片钳记录, 并突出了各方面的特点, 这是至关重要的成功。
Tags
Whole-cell Currents
GABA
Brain Slices
Puff Application
Electrophysiology
Pharmacological Administration
Acute Prefrontal Cortex Slice
Vibratome
ACSF
Recording Electrodes
Puff Micropipettes
Sodium Channel Blocker
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