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Journal
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Immunology and Infection
/
高通量并行测序在金黄叙利亚仓鼠感染期间测量
钩端螺旋体螺旋体
转插入突变体的适应性
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
High-throughput Parallel Sequencing to Measure Fitness of
Leptospira interrogans
Transposon Insertion Mutants During Golden Syrian Hamster Infection
Please note that all translations are automatically generated.
Click here for the English version.
高通量并行测序在金黄叙利亚仓鼠感染期间测量
钩端螺旋体螺旋体
转插入突变体的适应性
DOI:
10.3791/56442-v
•
11:50 min
•
December 18, 2017
•
Kristel Lourdault
2
,
James Matsunaga
2
,
Karen V. Evangelista
2
,
David A. Haake
2,3,4
1
Veterans Affairs Greater Los Angeles Healthcare System
,
2
Departments of Medicine
,
David Geffen School of Medicine at University of California Los Angeles
,
3
Departments of Urology
,
David Geffen School of Medicine at University of California Los Angeles
,
4
Departments of Microbiology, Immunology, and Molecular Genetics
,
University of California Los Angeles
Chapters
00:05
Title
01:17
Creation of the Transposon Mutant Library: Transfer of the Transposon into
Leptospira
spp. by Conjugation
03:56
Animal Experiment
04:57
Making the Genomic Library
09:24
Results: Challenged Hamsters
10:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们在这里描述的技术, 结合转诱变和高通量测序, 以确定和量化转端突变体在组织后的挑战, 仓鼠。该协议可用于筛选动物生存和传播的变种人, 也可用于
体外
研究。
Tags
High-throughput Sequencing
Transposon Insertion Mutants
Leptospira Interrogans
Golden Syrian Hamster Infection
In Vivo Fitness
In Vitro Fitness
Virulence Genes
Colonization
Dissemination
Survival
Filtration Unit
EMJH Plates
Kanamycin
Nested PCR
Transposon Insertion Site
Article
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