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高通量并行测序在金黄叙利亚仓鼠感染期间测量钩端螺旋体螺旋体转插入突变体的适应性
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
DOI:

11:50 min

December 18, 2017

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Chapters

  • 00:05Title
  • 01:17Creation of the Transposon Mutant Library: Transfer of the Transposon into Leptospira spp. by Conjugation
  • 03:56Animal Experiment
  • 04:57Making the Genomic Library
  • 09:24Results: Challenged Hamsters
  • 10:59Conclusion

Summary

Automatic Translation

我们在这里描述的技术, 结合转诱变和高通量测序, 以确定和量化转端突变体在组织后的挑战, 仓鼠。该协议可用于筛选动物生存和传播的变种人, 也可用于体外研究。

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