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Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
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JoVE Journal Immunology and Infection
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

DOI:
10.3791/56448-v

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12:09 min

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November 22, 2017

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, , , ,
1Influenza Division,Centers for Disease Control and Prevention, 2Battelle

Chapters

  • 00:05Title
  • 01:09Determination of Tissue-culture Infectious Dose (TCID) of the Virus
  • 05:44Microneutralization (MN) Assay Using MDCK-SIAT1 Cells
  • 09:44Results: Microneutralization Assay Results of Human Sera tested Against A/HongKong/4801/2014 A(H3N2) Virus Using MDCK-SIAT1 Cells
  • 11:25Conclusion

Summary

Automatic Translation

Automatic Translation

Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.

Tags

Keywords: InfluenzaNeutralizing AntibodyMicroneutralization AssayMDCK-SIAT1 CellsH3N2 VirusesVirus PropagationVirus DilutionELISANucleoprotein Monoclonal Antibody

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