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Journal
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Immunology and Infection
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慢性活性泼斯坦-巴尔病毒感染患者 NK 和 T 细胞系建立的高效简便方法
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Immunology and Infection
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JoVE Journal
Immunology and Infection
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
Please note that all translations are automatically generated.
Click here for the English version.
慢性活性泼斯坦-巴尔病毒感染患者 NK 和 T 细胞系建立的高效简便方法
DOI:
10.3791/56515-v
•
09:43 min
•
March 30, 2018
•
Chongfeng Xu
,
Junhong Ai
,
Qingxun Zhang
,
Ting Li
,
Xiaorong Wu
,
Zhengde Xie
,
Ziyuan Duan
1
Genetic Resources Center, Institute of Genetics and Developmental Biology
,
Chinese Academy of Sciences
,
2
MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital
,
Capital Medical University
,
3
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology
,
Chinese Academy of Sciences
Chapters
00:04
Title
01:35
Isolation of PBMC from CAEBV Patients
02:59
Expansion of Cells and Numbering the Viable Cells
04:08
Preparation for Cell Phenotyping by Flow Cytometry
05:52
Expansion and Cryopreservation of NK/T Cells
07:23
Results: Representative Gating Strategy for Flow Cytometry Analysis of Cell Lines L196 and M296
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
采用高效、少量外周血、低剂量 IL-2 的方法, 建立了 CAEBV 患者的 NK 和 T 细胞克隆的简便算法。
Tags
NK Cells
T Cells
Epstein-Barr Virus
CAEBV
Cell Line Establishment
Cell Culture
PBMC
IL-2
Cell Proliferation
Cell Viability
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