Journal
/
Immunology and Infection
/
Registrering og fjernelse af nukleasen forurening under oprensning af rekombinante Prototype skummende Virus Integrase
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Registrering og fjernelse af nukleasen forurening under oprensning af rekombinante Prototype skummende Virus Integrase

DOI:
10.3791/56605-v

•

07:50 min

•

December 08, 2017

•

, , ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Chapters

  • 00:05Title
  • 00:35Nickel Affinity Chromatography
  • 03:30Heparin Affinity Chromatography
  • 04:28Nuclease Assay
  • 06:46Results: Nuclease Assay of Heparin Sepharose Fractions
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

Rekombinant prototype skummende virus integrase protein er ofte forurenet med en bakteriel nukleasen under rensningen. Denne metode identificerer nukleasen forurening og fjerner det fra den endelige udarbejdelse af enzymet.

Tags

Nuclease ContaminationProtein PurificationRecombinant Prototype Foamy Virus IntegraseE. ColiSonicationUltracentrifugationFPLCNickel-charged ResinSDS-PAGEUV Absorbance

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Generation of Recombinant Influenza Virus from Plasmid DNA

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

High-throughput Detection Metode til Influenza Virus

Affinitetsrensning af Influenzavirus ribonukleoprotein komplekser fra kromatin af inficerede celler

In vivo Imaging Systems (IVIS) Påvisning af en neuro-Invasive encephalitiske Virus

Redning af rekombinant Newcastle Disease-virus fra cDNA

Hurtig screening af HIV revers transkriptase og Integrase hæmmere

Redning og karakterisering af rekombinant virus fra en ny verden Zika Virus infektiøs klon

Forsamling og rensning af Prototype skummende Virus Intasomes

Read Article