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人脑神经前体细胞神经发育表型的快速检测 (npc)
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
DOI:

10:47 min

March 02, 2018

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Chapters

  • 00:04Title
  • 01:08Collecting NPCs for Plating of Experimental Conditions
  • 02:30Neural Precursor Cell DNA Synthesis Assay
  • 03:47S Phase Entry Assay
  • 04:39Neural Precursor Cell Number Assay
  • 05:41Neural Precursor Cell Neurosphere Migration Assay
  • 07:58Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
  • 09:51Conclusion

Summary

Automatic Translation

神经发育过程, 如增殖, 迁移和突起的生长, 往往是不安的精神疾病。因此, 我们提出的协议, 快速和重现性评估这些神经发育过程中的人类 iPSC 派生的 npc。这些协议还允许评估相关生长因子和疗法对 NPC 发展的影响。

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