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JoVE Journal Neuroscience
Isolering og dyrkning af neurale stamceller efterfulgt af kromatin-Immunoprecipitation af Histon 3 lysin 79 Dimethylation Mark
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Isolering og dyrkning af neurale stamceller efterfulgt af kromatin-Immunoprecipitation af Histon 3 lysin 79 Dimethylation Mark

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Vi præsenterer en effektiv og reproducerbar metode til at isolere og kultur neurale stamceller fra embryonale og postnatal hjernevæv for kromatin immunoprecipitation (ChIP) af Histon 3 lysin 79 dimethylation (H3K79me2) - en Histon mark ligger inden for den kugleformede domæne af Histon 3.

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Neurovidenskab sag 131 cerebellare cellekultur kortikale cellekultur cortex udvikling cerebellare udvikling H3K79 methylering DOT1L ChIP DOT1L hæmning
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