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Journal
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Medicine
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小鼠尿的解剖和
外植体
培养对囊附着的作用分析
JoVE Journal
Medicine
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JoVE Journal
Medicine
Dissection and Explant Culture of Murine Allantois for the
In Vitro
Analysis of Allantoic Attachment
Please note that all translations are automatically generated.
Click here for the English version.
小鼠尿的解剖和
外植体
培养对囊附着的作用分析
DOI:
10.3791/56712-v
•
09:30 min
•
January 13, 2018
•
Kerstin Hadamek
2
,
Angelika Keller
2
,
Antje Gohla
2
1
Institute of Pharmacology and Toxicology
,
University of Würzburg
,
2
Rudolf Virchow Center for Experimental Biomedicine
,
University of Würzburg
Chapters
00:05
Title
00:42
Coating of Microtiter Plates with α4β1 Integrin for
Ex Utero
Allantois Explant Culture
01:54
Uterus Dissection from a Pregnent Mouse at DPC 8
03:24
Embryo and Allantois Dissection and
Ex Utero
Allantois Cultre
06:18
Staining Explanted Allantoides with Endothelial Cell Marker CD31
08:07
Results: Allantois Attachment and Immunohistochemistry
08:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个
体外
测定模型囊附着, 第一步胎盘形成。该协议展示了小鼠 allantoides 在固定化α4β1整合素上的解剖和外植体培养。尿附件是在预定的时间点显微镜下评估的。
Tags
Murine Allantois
Allantoic Attachment
Placenta Formation
Embryo Development
In Vitro Analysis
Alpha 4/beta 1 Integrin
Explant Culture
Dissection
Uterus
Mouse Embryo
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