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小鼠尿的解剖和外植体培养对囊附着的作用分析
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JoVE Journal Medicine
Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
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小鼠尿的解剖和外植体培养对囊附着的作用分析

DOI:
10.3791/56712-v

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09:30 min

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January 13, 2018

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, ,
1Institute of Pharmacology and Toxicology,University of Würzburg, 2Rudolf Virchow Center for Experimental Biomedicine,University of Würzburg

Chapters

  • 00:05Title
  • 00:42Coating of Microtiter Plates with α4β1 Integrin for Ex Utero Allantois Explant Culture
  • 01:54Uterus Dissection from a Pregnent Mouse at DPC 8
  • 03:24Embryo and Allantois Dissection and Ex Utero Allantois Cultre
  • 06:18Staining Explanted Allantoides with Endothelial Cell Marker CD31
  • 08:07Results: Allantois Attachment and Immunohistochemistry
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个体外测定模型囊附着, 第一步胎盘形成。该协议展示了小鼠 allantoides 在固定化α4β1整合素上的解剖和外植体培养。尿附件是在预定的时间点显微镜下评估的。

Tags

Murine AllantoisAllantoic AttachmentPlacenta FormationEmbryo DevelopmentIn Vitro AnalysisAlpha 4/beta 1 IntegrinExplant CultureDissectionUterusMouse Embryo

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