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Visualisera intracellulära SNARA människohandel genom fluorescens livstid Imaging mikroskopi
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
DOI:

08:55 min

December 29, 2017

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Chapters

  • 00:05Title
  • 00:37Preparation of the Microscopy Samples
  • 01:49Recording of the FLIM Data
  • 03:34Conversion of the Photon Recordings to FLIM Images
  • 04:19Fitting of Fluorescence Lifetime Histograms for Whole-cell FLIM Analysis
  • 06:03Results: Syntaxin 4 and VAMP3 Interaction in Live Cell Visualized by FRET-FLIM
  • 07:43Conclusion

Summary

Automatic Translation

Det här protokollet beskriver en ny metod som möjliggör kvantitativa visualisering av komplexa bildandet av SNARE proteiner, baserat på Förster resonans energiöverföring och fluorescens livstid imaging mikroskopi.

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