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Journal
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Developmental Biology
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果蝇
蛹卵巢的解剖与染色
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Dissection and Staining of
Drosophila
Pupal Ovaries
Please note that all translations are automatically generated.
Click here for the English version.
果蝇
蛹卵巢的解剖与染色
DOI:
10.3791/56779-v
•
07:35 min
•
March 02, 2018
•
Karen Sophia Park
,
Dorothea Godt
,
Daniel Kalderon
1
Department of Biological Sciences
,
Columbia University
,
2
Department of Cell and Systems Biology
,
University of Toronto
Chapters
00:05
Title
00:41
Egg Laying and Female Larvae Selection
02:12
Pupae Antibody Staining Preparation
04:24
Immunohistochemistry
05:52
Dissecting and Mounting Pupal Ovaries
06:49
Results: Clear Antibody Staining that Reveals
Drosophila
Structure and Cellular Organization
07:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
果蝇
卵巢是研究干细胞小生境发育的优良模型系统。虽然已经公布了解剖幼虫和成人卵巢的方法, 蛹卵巢解剖需要不同的技术, 没有详细发表。在这里, 我们概述了解剖, 染色和安装蛹卵巢的协议。
Tags
Drosophila
Pupal Ovaries
Dissection
Staining
Stem Cell Differentiation
Time Course Experiments
Stem Cell Division
Wandering Third Instar Larvae
Phosphate Buffered Saline (PBS)
Male And Female Larvae
Pupariation
Pre-pupa
Glass Pipette Tip
Protein Glue
Article
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