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一种基于动力学荧光的 Ca
2 +
动员法, 用于识别 G 蛋白偶联受体激动剂、拮抗器和构调制器
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JoVE Journal
Medicine
A Kinetic Fluorescence-based Ca
2+
Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Please note that all translations are automatically generated.
Click here for the English version.
一种基于动力学荧光的 Ca
2 +
动员法, 用于识别 G 蛋白偶联受体激动剂、拮抗器和构调制器
DOI:
10.3791/56780-v
•
07:41 min
•
February 20, 2018
•
Sandra Claes
,
Thomas D’huys
,
Anneleen Van Hout
,
Dominique Schols
,
Tom Van Loy
1
Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research
,
KU Leuven
Chapters
00:05
Title
00:50
Ca
2+
Mobilization Assay Cell Seeding
01:58
Fluorescence Plate Reader Protocol Setup
04:38
Fluorescence Assay
05:36
Results: Representative Chemokine Ligand: Receptor Specificity Analyses
07:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
所描述的细胞检测是为鉴定 CXC 趋化因子受体 4 (CXCR4)-相互作用的代理, 抑制或刺激, 无论是竞争性或 allosterically, 细胞内 Ca
2 +
释放启动的 CXCR4 激活。
Tags
G Protein-coupled Receptor
Calcium Mobilization
Fluorescence-based Assay
Agonist
Antagonist
Allosteric Modulator
Drug Discovery
Intracellular Calcium
GPCR
Cell-based Assay
High-throughput Screening
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