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Journal
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Biochemistry
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核糖体剖面对芽殖酵母中翻译的全基因组定量
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
Please note that all translations are automatically generated.
Click here for the English version.
核糖体剖面对芽殖酵母中翻译的全基因组定量
DOI:
10.3791/56820-v
•
12:57 min
•
December 21, 2017
•
Carine Beaupere
,
Rosalyn B. Chen
,
William Pelosi
,
Vyacheslav M. Labunskyy
1
Department of Dermatology
,
Boston University School of Medicine
Chapters
00:05
Title
00:52
Extract Preparation
02:43
Footprint Extraction
05:15
Dephosphorylation
07:07
Reverse Transcription
10:17
Results: Ribo-seq Enables Genome-wide Quantification of Translation of Specific mRNAs Under Different Cellular Conditions
11:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
翻译调控在蛋白质丰度控制中起着重要的作用。在这里, 我们描述一个高通量的方法, 定量分析翻译在萌芽酵母
酿酒
酵母。
Tags
Ribosome Profiling
Genome-wide
Translation
Budding Yeast
Saccharomyces Cerevisiae
Protein Translation
Translational Regulation
Cell Lysate
Poly(A) MRNA
Sucrose Gradient
RNase I
Cycloheximide
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