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Journal
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Biochemistry
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用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学
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Biochemistry
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JoVE Journal
Biochemistry
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
Please note that all translations are automatically generated.
Click here for the English version.
用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学
DOI:
10.3791/56846-v
•
10:41 min
•
March 07, 2018
•
Bo Han
,
Man Zhang
,
Peng Sun
,
Shangwei Hou
1
Department of General Surgery, Tongren Hospital
,
Shanghai Jiao Tong University School of Medicine
,
2
Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine
,
Shanghai Jiao Tong University
,
3
Hongqiao International Institute of Medicine, Tongren Hospital
,
Shanghai Jiao Tong University School of Medicine
Chapters
00:04
Title
01:01
Affinity Purification of FLAG-tagged hEAG1 Channel Protein from HEK-293 Cells
04:18
BCA Assay, Western Blotting, and Labeling the Purified Protein with Biotin for the BLI Assay
06:10
Bio-layer Interferometry Assay
08:06
Results: Interaction Kinetics of hEAG1 with PIP2 Using the Bio-layer Interferometry Assay
09:35
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了纯化 hEAG1 离子通道蛋白与小分子脂配体磷酸肌醇4、5-bisphosphate (
PIP
2) 的相互作用。实验表明, BLI 可能是一种新的小分子离子通道配体筛选的潜在方法。
Tags
Bio-layer Interferometry
BLI Assay
Ion Channel
Protein-small Molecule Interaction
Kinetics
HEAG1
Cell Culture
Protein Expression
Purification
Affinity Chromatography
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