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Absolut kvantificering af mikroRNA plasmaniveauer i Cynomolgus aber, ved hjælp af kvantitative Real-time Reverse transkription PCR
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Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Absolut kvantificering af mikroRNA plasmaniveauer i Cynomolgus aber, ved hjælp af kvantitative Real-time Reverse transkription PCR

DOI:
10.3791/56850-v

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10:23 min

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February 12, 2018

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, , , ,
1Medicinal Safety Research Laboratories,Daiichi Sankyo Co., Ltd.

Chapters

  • 00:04Title
  • 01:01RNA Extraction
  • 03:43cDNA Synthesis
  • 04:55Pre-amplification
  • 06:02Quantitative Real-time PCR
  • 07:05Data Analysis
  • 07:27Results: Use of Quantitative Real-time Reverse Transcription PCR with or without Pre-amplification to Measure the Absolute Levels of Plasma micro-RNAs
  • 09:54Conclusion

Summary

Automatic Translation

Automatic Translation

Denne rapport beskriver en protokol til måling af de absolutte niveauer af plasma miRNA, ved hjælp af kvantitative real-time reverse transkription PCR, med eller uden forudgående forstærkning. Denne protokol giver bedre forståelse af mængden af plasma miRNAs og giver mulighed for kvalitativ vurdering af tilsvarende data fra forskellige undersøgelser eller laboratorier.

Tags

Plasma MicroRNAAbsolute QuantificationQuantitative Real-time Reverse Transcription PCRCynomolgus MonkeysRNA ExtractionSynthetic MicroRNABiomarkersTranslational Research

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