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En manipuleret Split-TET2 enzym til kemikalie-inducerbar DNA Hydroxymethylation og epigenetiske Remodeling
JoVE Journal
Chemistry
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JoVE Journal Chemistry
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
DOI:

08:34 min

December 18, 2017

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Chapters

  • 00:05Title
  • 00:48Cell Culture, Plasmid Transfection, and Chemical Induction
  • 01:45Quantification of Rapamycin-indiced 5-hydroxymethylcystosine (5hmc) Production by Immunostaining
  • 03:08Dot-blot Assay Quantify 5hmc and 5-methylcystosine (5mc) Amounts
  • 07:03Results: Validation of Chemically Induced 5hmc Levels
  • 07:58Conclusion

Summary

Automatic Translation

Detaljerede protokoller for at indføre et manipuleret split-TET2 enzym (CiDER) i pattedyrceller for kemiske inducerbar DNA hydroxymethylation og epigenetiske remodeling præsenteres.

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