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用触印细胞学方法快速获得临床病理标本中高质量肿瘤 DNA
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Cancer Research
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JoVE Journal Cancer Research
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
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用触印细胞学方法快速获得临床病理标本中高质量肿瘤 DNA

DOI:
10.3791/56943-v

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11:20 min

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March 21, 2018

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, , ,
1Genome Analysis Center,Yamanashi Central Hospital, 2Pathology Division, Laboratory Department,Yamanashi Central Hospital, 3The University of Tokyo

Chapters

  • 00:04Title
  • 00:37TIC Preparation for Quick Microscopic Assessment
  • 02:04PEN Membrane Slide Film Preparation for Genetic Testing
  • 02:40Next Generation Sequencing
  • 09:24Results: TIC DNA Suitable for Range of Genetic Testing
  • 10:57Conclusion

Summary

Automatic Translation

Automatic Translation

从肿瘤组织获得高质量的基因组 DNA 是利用下一代测序分析基因改变的重要第一步。本文提出一种简便、快速的方法来丰富肿瘤细胞, 从接触印记细胞学标本中获得完整的 DNA。

Tags

Tumor DNATouch Imprint CytologyClinical-pathological SpecimensHigh-quality DNARapid DNA ExtractionCancer ResearchDiagnosisTreatmentDNA ExtractionMicroscopic AssessmentDNA Sequencing

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