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Journal
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Developmental Biology
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人間の腸 Organoids 上皮関門の透過性のリアルタイム計測
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
Please note that all translations are automatically generated.
Click here for the English version.
人間の腸 Organoids 上皮関門の透過性のリアルタイム計測
DOI:
10.3791/56960-v
•
08:04 min
•
December 18, 2017
•
David R. Hill
,
Sha Huang
,
Yu-Hwai Tsai
,
Jason R. Spence
2
,
Vincent B. Young
3
1
Department of Internal Medicine, Division of Gastroenterology
,
University of Michigan
,
2
Department of Cell and Developmental Biology
,
University of Michigan
,
3
Department of Internal Medicine, Division of Infectious Disease
,
University of Michigan
Chapters
00:05
Title
00:45
Sterile Microinjection
04:07
Pharmacological Treatment of HIOs
05:13
Results: Epithelial Barrier Permeability in HIOs is Affected by Bacterial Toxin
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、蛍光顕微鏡を用いたヒトの腸オルガノイドのリアルタイム次の薬理学的治療における上皮バリア透過性の測定について説明し、細胞顕微鏡検査をライブします。
Tags
Epithelial Barrier Permeability
Human Intestinal Organoids
Real-time Measurement
FITC-dextran
Microinjection
Gastroenterology
Toxins
Bacterial Products
Pharmacologic Treatments
Visualization
Quantitative Measure
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