Journal
/
Neuroscience
/
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

DOI:
10.3791/57015-v

•

07:05 min

•

January 19, 2018

•

,
1Department of Genetics and Evolution,University of Geneva

Chapters

  • 00:05Title
  • 00:40Preparations and Dissection
  • 02:48Embedding Brain Explants in a 3D Matrix and Mounting
  • 04:56Results: Long-term Recordings of Circadian Fluorescent Reports in Ex Vivo Larval Brain Cultures
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to establish ex vivo Drosophila larval brain culture optimized to monitor circadian molecular rhythms with long-term fluorescence time-lapse imaging. The application of this method to pharmacological assays is also discussed.

Tags

Single-cellFluorescenceLive ImagingDrosophilaCircadian ClocksLarval Brain CultureNeurogeneticsGene ExpressionFluorescent ProbesSAM MediumThrombinPenicillin StreptomycinFibrinogenDissectionForcepsDSSThird Instar LarvaeBrain TransferEmbedding Procedure

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Super-resolution Imaging of Neuronal Dense-core Vesicles

Read Article