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Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
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Neuroscience
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JoVE Journal Neuroscience
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

DOI:
10.3791/57015-v

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07:05 min

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January 19, 2018

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,
1Department of Genetics and Evolution,University of Geneva

Chapters

  • 00:05Title
  • 00:40Preparations and Dissection
  • 02:48Embedding Brain Explants in a 3D Matrix and Mounting
  • 04:56Results: Long-term Recordings of Circadian Fluorescent Reports in Ex Vivo Larval Brain Cultures
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to establish ex vivo Drosophila larval brain culture optimized to monitor circadian molecular rhythms with long-term fluorescence time-lapse imaging. The application of this method to pharmacological assays is also discussed.

Tags

Keywords: Single-cellFluorescenceLive ImagingDrosophilaCircadian ClocksLarval Brain CultureNeurogeneticsGene ExpressionFluorescent ProbesSAM MediumThrombinPenicillin StreptomycinFibrinogenDissectionForcepsDSSThird Instar LarvaeBrain TransferEmbedding Procedure

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