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单细胞分辨荧光活成像在幼虫脑培养中的果蝇昼夜节律时钟
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Neuroscience
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JoVE Journal Neuroscience
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
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单细胞分辨荧光活成像在幼虫脑培养中的果蝇昼夜节律时钟

DOI:
10.3791/57015-v

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07:05 min

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January 19, 2018

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,
1Department of Genetics and Evolution,University of Geneva

Chapters

  • 00:05Title
  • 00:40Preparations and Dissection
  • 02:48Embedding Brain Explants in a 3D Matrix and Mounting
  • 04:56Results: Long-term Recordings of Circadian Fluorescent Reports in Ex Vivo Larval Brain Cultures
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目的是建立活体果蝇幼虫脑培养优化监测昼夜节律分子节律与长期荧光时间推移成像。并对该方法在药理实验中的应用进行了探讨。

Tags

Keywords: Single-cellFluorescenceLive ImagingDrosophilaCircadian ClocksLarval Brain CultureNeurogeneticsGene ExpressionFluorescent ProbesSAM MediumThrombinPenicillin StreptomycinFibrinogenDissectionForcepsDSSThird Instar LarvaeBrain TransferEmbedding Procedure

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