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January 19, 2018
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Summary
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Målet med detta protokoll är att etablera ex vivo Drosophila larval hjärnan kultur optimerad för att övervaka dygnsrytm molekylär rytmer med långsiktiga fluorescens time-lapse imaging. Tillämpningen av denna metod på farmakologiska analyser diskuteras också.
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Cite this Article
Sabado, V., Nagoshi, E. Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture. J. Vis. Exp. (131), e57015, doi:10.3791/57015 (2018).
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