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Journal
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Cancer Research
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利用小鼠脑肿瘤球的原发染色质沉淀
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Native Chromatin Immunoprecipitation Using Murine Brain Tumor Neurospheres
Please note that all translations are automatically generated.
Click here for the English version.
利用小鼠脑肿瘤球的原发染色质沉淀
DOI:
10.3791/57016-v
•
09:31 min
•
January 29, 2018
•
Flor M. Mendez
,
Felipe J. Núñez
2
,
Rocío I. Zorrilla-Veloz
4
,
Pedro R. Lowenstein
2
,
Maria G. Castro
2
1
Department of Cell and Developmental Biology
,
University of Michigan Medical School
,
2
Department of Neurosurgery
,
University of Michigan Medical School
,
3
Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program
,
University of Michigan Medical School
,
4
Department of Biology
,
University of Puerto Rico-Río Piedras Campus
Chapters
00:05
Title
00:40
Generation of Brain Tumor Neurospheres and Culturing Conditions
02:23
Chromatin Preparation
04:52
Immunoprecipitation (IP)
08:21
Results: Representative ChIP qPCR Data as Percent Input
09:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
后生机制在胶质瘤中经常被改变。染色质沉淀可用于研究基因改变的后果, 胶质瘤的改变导致的组蛋白修饰, 调节染色质结构和基因转录。本协议描述了小鼠脑肿瘤球的原发染色质沉淀。
Tags
Native Chromatin Immunoprecipitation
Murine Brain Tumor
Neurospheres
Histone Marks
Epigenetics
Tumor Cells Transcriptome
Cross-linking
Antibody Specificity
Fluorescent Protein
Cell Detachment
Neurosphere Formation
Micrococcal Nuclease
Chromatin Digestion
Immunoprecipitation
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