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杆状病毒基因治疗应用的生产与纯化

JoVE Journal
Genetics

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JoVE Journal Genetics

Production and Purification of Baculovirus for Gene Therapy Application

Please note that all translations are automatically generated. Click here for the English version.

杆状病毒基因治疗应用的生产与纯化

DOI:

10.3791/57019-v

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06:11 min

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April 09, 2018

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Md Nasimuzzaman², Johannes C.M. van der Loo^2,3, Punam Malik²

¹Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, ²University of Cincinnati College of Medicine, ³Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia

Chapters

00:04Title
00:55Propagation and Harvest of Baculovirus
02:20Purification of Baculovirus Vector
04:00Concentration of Baculovirus
05:09Results: The Yield of Infectious Baculovirus During Heparin Affinity Chromatography
05:42Conclusion

Summary

Automatic Translation

在该协议中, 杆状病毒是通过瞬态转染杆状病毒质粒进入 Sf9 细胞并在无血清悬浮培养中扩增而产生的。用肝素亲和层析法纯化上清液, 进一步浓缩离心。该协议对用于基因治疗的杆状病毒的制备和纯化具有重要意义。

Tags

AI-based Gene Therapy Baculovirus Production Baculovirus Purification Heparin Affinity Chromatography Ultracentrifugation Sf9 Cell Culture Viral Vector Gene Transfer Vaccine Development

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