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Journal / Developmental Biology / Kinetic analyse af Vasculogenesis kvantificerer…
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Developmental Biology

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JoVE Journal Developmental Biology
Kinetic analyse af Vasculogenesis kvantificerer dynamikken i Vasculogenesis og angiogenese In Vitro
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Kinetic analyse af Vasculogenesis kvantificerer dynamikken i Vasculogenesis og angiogenese In Vitro

Article DOI: 10.3791/57044-v • 11:03 min • January 31st, 2018
January 31st, 2018 •
1,2, 3,4, 3,4, 1,2,5,6,7
1Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 3Indiana Center for Biological Microscopy, Indiana University School of Medicine, 4Department of Medicine, Indiana University School of Medicine, 5Department of Pediatrics, Indiana University School of Medicine, 6Department of Microbiology and Immunology, Indiana University School of Medicine, 7Indiana University Simon Cancer Center, Indiana University School of Medicine

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Her præsenterer vi en protokol for time-lapse billedbehandling og analyse af vasculogenesis i vitro bruger fase kontrast mikroskopi kombineret med open source-software, Kinetic analyse af Vasculogenesis. Denne protokol kan anvendes til at vurdere kvantitativt vasculogenic af mange celletyper eller forsøgsbetingelser, at model vaskulær sygdom.

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Udviklingsmæssige biologi sag 131 Vasculogenesis endotel kolonidannende celler mikroskopi svangerskabsdiabetes time-lapse imaging kinetic netværk dannelse
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