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Kinetic analyse af Vasculogenesis kvantificerer dynamikken i Vasculogenesis og angiogenese In Vitro
This content is Free Access.
1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine
Chapters
- 00:05Title
- 00:59Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
- 04:46Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
- 08:12Results: Representative Kinetic Analysis of Vasculogenesis
- 10:22Conclusion
Her præsenterer vi en protokol for time-lapse billedbehandling og analyse af vasculogenesis i vitro bruger fase kontrast mikroskopi kombineret med open source-software, Kinetic analyse af Vasculogenesis. Denne protokol kan anvendes til at vurdere kvantitativt vasculogenic af mange celletyper eller forsøgsbetingelser, at model vaskulær sygdom.
