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A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
A Simple and Efficient Method for <em>In Vivo</em> Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
JoVE Journal
Biology
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JoVE Journal Biology
A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

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06:42 min

April 16, 2018

DOI:

06:42 min
April 16, 2018

16000 Views
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Summary

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Here we present a protocol for cardiac-specific gene manipulation in mice. Under anesthesia, the mouse hearts were externalized through the fourth intercostal space. Subsequently, adenoviruses encoding specific genes were injected with a syringe into the myocardium, followed by protein expression measurement via in vivo imaging and Western blot analysis.

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