A Simple and Efficient Method for <em>In Vivo</em> Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

Yanan Fu; Wenlong Jiang; Yichao Zhao; Yuli Huang; Heng Zhang; Hongju Wang; Jun Pu

doi:10.3791/57074

Journal

/

Biology

/

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

A Simple and Efficient Method for <em>In Vivo</em> Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

16,034 Views

•

06:42 min

•

April 16, 2018

DOI:

10.3791/57074-v

DOI:

10.3791/57074-v

•

06:42 min

April 16, 2018

•

16000 Views

Yanan Fu*^1,2, Wenlong Jiang*², Yichao Zhao*², Yuli Huang¹, Heng Zhang¹, Hongju Wang¹, Jun Pu^1,2

¹Department of Cardiology,The First Affiliated Hospital of Bengbu Medical College ²Department of Cardiology, Ren Ji Hospital, School of Medicine,Shanghai Jiao Tong University

Summary

Here we present a protocol for cardiac-specific gene manipulation in mice. Under anesthesia, the mouse hearts were externalized through the fourth intercostal space. Subsequently, adenoviruses encoding specific genes were injected with a syringe into the myocardium, followed by protein expression measurement via in vivo imaging and Western blot analysis.