Here we present a protocol for cardiac-specific gene manipulation in mice. Under anesthesia, the mouse hearts were externalized through the fourth intercostal space. Subsequently, adenoviruses encoding specific genes were injected with a syringe into the myocardium, followed by protein expression measurement via in vivo imaging and Western blot analysis.
Fu, Y., Jiang, W., Zhao, Y., Huang, Y., Zhang, H., Wang, H., Pu, J. A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice. J. Vis. Exp. (134), e57074, doi:10.3791/57074 (2018).