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Isolering af mesenkymale stamceller fra menneskelige alveolær Periosteum og effekter af D-Vitamin på Osteogenic aktivitet af Periosteum-afledte celler
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
DOI:

06:47 min

May 04, 2018

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Chapters

  • 00:05Title
  • 01:10Tissue Preparations
  • 03:15Cell Attachment and Viability with Osteogenic Differentiation
  • 04:28Results: Osteogenic Differentiation of PDCs and Analysis of 1,25-dihydroxy Vitamin D (Calcitriol) and Vitamin C Treatment of PDCs using RT-PCR
  • 06:11Conclusion

Summary

Automatic Translation

Vi præsenterer en protokol for at undersøge mRNA udtryk biomarkører periosteum-afledte celler (fremdaterede) foranlediget af vitamin C (C-vitamin) og 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. Derudover vurderer vi fremdaterede evne til at differentiere i osteocytes, chondrocytter og adipocytter.

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