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Replikering af bestilt, Nonredundant bibliotek af Pseudomonas aeruginosa stamme PA14 Transposon indsættelse mutanter
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Immunology and Infection
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JoVE Journal Immunology and Infection
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Replikering af bestilt, Nonredundant bibliotek af Pseudomonas aeruginosa stamme PA14 Transposon indsættelse mutanter

DOI:
10.3791/57298-v

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11:35 min

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May 04, 2018

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, , , , , , ,
1Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, 2Department of Molecular Biology,Massachusetts General Hospital, 3Department of Genetics,Harvard Medical School, 4Department of Pediatrics,Harvard Medical School

Chapters

  • 00:04Title
  • 01:26Growth of PA14NR Set Master Copy on LB Agar Plates
  • 05:02Growth of the PA14NR Set Master Copy in LB Liquid Broth
  • 07:01Transfer of PA14NR Set Overnight Cultures into Destination Plates
  • 09:43Results: Protocol Generates High-quality Copies of the PA14NR Set Mutant Library
  • 10:38Conclusion

Summary

Automatic Translation

Automatic Translation

Pseudomonas aeruginosa infektion forårsager betydelig sygelighed i sårbare værter. Nonredundant transposon indsættelse mutant bibliotek af P. aeruginosa stamme PA14, udpeget som PA14NR sæt, letter analyse af genet funktionalitet i mange processer. Præsenteres her er en protokol til at skabe høj kvalitet kopier af PA14NR indstillet mutant biblioteket.

Tags

Pseudomonas AeruginosaPA14NR SetTransposon Mutant LibraryReplicationQuality ControlContamination PreventionSterile TechniquesReplicator Pin SterilizationLB Agar PlatesFreezer Storage

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