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Journal
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Biochemistry
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利用分子荧光互补的方法照亮 Caspase 活化的途径
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
Please note that all translations are automatically generated.
Click here for the English version.
利用分子荧光互补的方法照亮 Caspase 活化的途径
DOI:
10.3791/57316-v
•
08:47 min
•
March 05, 2018
•
Chloé I Charendoff
,
Lisa Bouchier-Hayes
1
Department of Pediatrics, Division of Hematology-Oncology
,
Baylor College of Medicine
,
2
Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology
,
Baylor College of Medicine
Chapters
00:04
Title
00:36
Transfection of Cells to Introduce the Caspase Bimolecular Fluorescence Complementation (BiFC) Components
02:42
Three-dimensional Imaging of Caspase BiFC
05:00
Time-lapse Imaging of Caspase BiFC
06:19
Results: Visualization of Caspase Activation Pathways Using Caspase Bimolecular Fluorescence Complementation
07:37
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述 caspase 分子荧光互补 (BiFC);一种基于图像的方法, 可用于可视化引发器半胱氨酸蛋白酶的诱导接近度, 这是它们激活的第一步。
Tags
Caspase Activation
Bimolecular Fluorescence Complementation
Induced Proximity
Apoptotic Cell Death
Initiator Caspases
Transfection
Reporter Plasmid
BiFC Plasmid
DNA Lipid Complex
Microscopy
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