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Um rastreio de baseada em célula de murino ilhotas pancreáticas para produtos químicos ambientais Diabetogenic
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
DOI:

07:39 min

June 25, 2018

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Chapters

  • 00:04Title
  • 00:49Pancreas Perfusion and Removal
  • 01:43Pancreas Digestion and Purification of Islets
  • 03:30Plating and Treatment of Islet Cells
  • 04:23ROS Staining and Measurement
  • 05:28Results: Xenobiotic Treatment-induced Reactive Oxygen Species and Inhibition of Insulin Secretion in Isolated Pancreatic Islet Cells
  • 06:39Conclusion

Summary

Automatic Translation

Aqui nós apresentamos um protocolo para isolar células de ilhotas pancreáticas do mouse para as induções ROS de triagem pelos xenobióticos para identificar os potenciais químicos xenobióticos de diabetogenic.

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