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En Microfluidic plattform for langsgående Imaging i Caenorhabditis elegans
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Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

En Microfluidic plattform for langsgående Imaging i Caenorhabditis elegans

DOI:
10.3791/57348-v

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09:00 min

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May 02, 2018

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,
1Department of Physics Education,Kongju National University, 2Department of Physics,Harvard University, 3FAS Center for Systems Biology,Harvard University

Chapters

  • 00:04Title
  • 00:58Microfluidic Environment Preparation
  • 03:35Microfluidic Device Bacteria Loading
  • 04:53Microfluidic Device Worm Loading
  • 06:50Results: Representative Gene Expression and Egg Laying Kinetics
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

I denne artikkelen viser vi live bildebehandling av personlige ansette en egendefinert microfluidic enhet. I enheten, er flere ormer individuelt begrenset for å skille chambers, slik at multiplex langsgående overvåking av ulike biologiske prosesser.

Tags

Microfluidic PlatformCaenorhabditis ElegansLongitudinal ImagingEnvironmental ConditionsWorm Behavioral ResponsesGene ExpressionAdaptationMathematical ModelingHypothesis TestingSyringe TubeAdapter NeedleBuffer ExchangeS-mediumDevice OutletBuffer InletWorm Inlet

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