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Opførelsen af syntetiske Phage vises Fab bibliotek med skræddersyede mangfoldighed
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
DOI:

12:31 min

May 01, 2018

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Chapters

  • 00:04Title
  • 01:18Electroporation and Calculation of the Library Size
  • 06:02Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
  • 09:43Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
  • 11:07Conclusion

Summary

Automatic Translation

Denne protokol beskriver en detaljeret procedure for opførelsen af en phage-vises syntetisk antistof bibliotek med skræddersyede mangfoldighed. Syntetisk antistoffer har bredt programmer fra grundforskning til sygdom diagnostik og terapi.

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