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Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
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Immunology and Infection
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JoVE Journal Immunology and Infection
Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

DOI:
10.3791/56401-v

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08:04 min

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December 20, 2017

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, , , ,
1Department of Medicine, Division of Regenerative Medicine,Loma Linda University

Chapters

  • 00:05Title
  • 00:56Overlapping Peptide (OLP) Library Generation and T Cell Priming with OLP Pool-pulsed Dendritic Cells (DCs) Protocol
  • 03:43Re-stimulation of OLP Pool-primed CD8+ T Cells Using Macrophages Pulsed with the OLP Pool
  • 06:19Results: Identification of a 9-mer Qa-1 Epitope in Myelin Oligodendrocyte Glycoprotein (MOG)
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Qa-1 (HLA-E in human) belongs to a group of non-classical major histocompatibility complex 1b molecules. Immunization with Qa-1-binding epitopes has been shown to augment tissue-specific immune regulation and ameliorate several autoimmune diseases. Herein we describe an overlapping peptide library strategy for the identification of Qa-1 epitopes in a protein.

Tags

Keywords: Overlapping Peptide LibraryQa-1 EpitopesImmune RegulationImmunotherapyMultiple SclerosisHLA-EDendritic CellsCD8+ T CellsInterleukin 2Interleukin 7

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