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长期活细胞成像对紫杉醇反应细胞命运的评估
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Cancer Research
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JoVE Journal Cancer Research
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
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长期活细胞成像对紫杉醇反应细胞命运的评估

DOI:
10.3791/57383-v

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08:29 min

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May 14, 2018

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, ,
1Department of Pharmacology & Experimental Therapeutics,Boston University School of Medicine, 2Department of Medicine, Section of Hematology and Oncology,Boston University School of Medicine

Chapters

  • 00:04Title
  • 00:39Setting Up the Microscope for Live-cell Imaging
  • 02:47Video Analysis to Identify Cell Fate in Response to Paclitaxel
  • 04:32Video Analysis to Identify Cell Fate Following Mitotic Slippage
  • 06:11Video Analysis to Identify Frequency of Nuclear Envelope Rupture
  • 07:13Results: Cell Fate Responses to Paclitaxel Treatment
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

活细胞成像提供了丰富的信息, 单个细胞或整个人口, 是无法实现的固定细胞成像单独。这里描述了用抗有丝分裂药物紫杉醇治疗后细胞命运决定的活细胞成像协议。

Tags

Live-cell ImagingCell FatePaclitaxelAnti-mitotic DrugMicroscopyKohler IlluminationAuto-focusTime-lapse ImagingCell Confluence

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