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Cancer Research
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长期活细胞成像对紫杉醇反应细胞命运的评估
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Cancer Research
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JoVE Journal
Cancer Research
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
Please note that all translations are automatically generated.
Click here for the English version.
长期活细胞成像对紫杉醇反应细胞命运的评估
DOI:
10.3791/57383-v
•
08:29 min
•
May 14, 2018
•
Amanda F. Bolgioni
,
Marc A. Vittoria
,
Neil J. Ganem
2
1
Department of Pharmacology & Experimental Therapeutics
,
Boston University School of Medicine
,
2
Department of Medicine, Section of Hematology and Oncology
,
Boston University School of Medicine
Chapters
00:04
Title
00:39
Setting Up the Microscope for Live-cell Imaging
02:47
Video Analysis to Identify Cell Fate in Response to Paclitaxel
04:32
Video Analysis to Identify Cell Fate Following Mitotic Slippage
06:11
Video Analysis to Identify Frequency of Nuclear Envelope Rupture
07:13
Results: Cell Fate Responses to Paclitaxel Treatment
08:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
活细胞成像提供了丰富的信息, 单个细胞或整个人口, 是无法实现的固定细胞成像单独。这里描述了用抗有丝分裂药物紫杉醇治疗后细胞命运决定的活细胞成像协议。
Tags
Live-cell Imaging
Cell Fate
Paclitaxel
Anti-mitotic Drug
Microscopy
Kohler Illumination
Auto-focus
Time-lapse Imaging
Cell Confluence
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