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多拷贝产质粒在抗生素耐药性演变中的作用试验

JoVE Journal
Genetics

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JoVE Journal Genetics

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

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多拷贝产质粒在抗生素耐药性演变中的作用试验

DOI:

10.3791/57386-v

•

09:00 min

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May 02, 2018

•

Jose Antonio Escudero², R Craig MacLean, Alvaro San Millan

¹Department of Animal Health,Universidad Complutense de Madrid, ²Visavet Health Surveillance Centre,Universidad Complutense de Madrid, ³Department of Zoology,University of Oxford, ⁴Department of Microbiology,Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

Chapters

00:05Title
00:33Construction of the Experimental System Encoding the Antibiotic Resistance Gene
04:19Evolutionary Rescue Approach to Experimentally Evolve Antibiotic Resistance
06:14Molecular Basis of the Evolution of Antibiotic Resistance
07:05Results: Study of Evolutionary Rescue and Molecular Basis Underlying the Plasmid Mediated Evolution using DNA Sequencing
08:35Conclusion

Summary

Automatic Translation

在此, 我们提出了一个实验方法来检验多拷贝产质粒在抗生素耐药性进化中的作用。

Tags

Keywords: Antibiotic Resistance Multicopy Plasmids Experimental Method Microbiology Bacterial Evolution Molecular Biology PCR Isothermal Assembly Electroporation Antibiotic Selection DNA Sequencing Plasmid Construction

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