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多拷贝产质粒在抗生素耐药性演变中的作用试验
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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
Please note that all translations are automatically generated.
Click here for the English version.
多拷贝产质粒在抗生素耐药性演变中的作用试验
DOI:
10.3791/57386-v
•
09:00 min
•
May 02, 2018
•
Jose Antonio Escudero
2
,
R Craig MacLean
,
Alvaro San Millan
1
Department of Animal Health
,
Universidad Complutense de Madrid
,
2
Visavet Health Surveillance Centre
,
Universidad Complutense de Madrid
,
3
Department of Zoology
,
University of Oxford
,
4
Department of Microbiology
,
Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP
Chapters
00:05
Title
00:33
Construction of the Experimental System Encoding the Antibiotic Resistance Gene
04:19
Evolutionary Rescue Approach to Experimentally Evolve Antibiotic Resistance
06:14
Molecular Basis of the Evolution of Antibiotic Resistance
07:05
Results: Study of Evolutionary Rescue and Molecular Basis Underlying the Plasmid Mediated Evolution using DNA Sequencing
08:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在此, 我们提出了一个实验方法来检验多拷贝产质粒在抗生素耐药性进化中的作用。
Tags
Antibiotic Resistance
Multicopy Plasmids
Experimental Method
Microbiology
Bacterial Evolution
Molecular Biology
PCR
Isothermal Assembly
Electroporation
Antibiotic Selection
DNA Sequencing
Plasmid Construction
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