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用无标签荧光显微镜评价活体、人体阻力动脉中胶原蛋白和弹性蛋白的压力依赖性 Microarchitectures
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
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用无标签荧光显微镜评价活体、人体阻力动脉中胶原蛋白和弹性蛋白的压力依赖性 Microarchitectures

DOI:
10.3791/57451-v

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09:58 min

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April 09, 2018

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, , ,
1Department of Cardiovascular and Renal Research, Institute of Molecular Medicine,University of Southern Denmark, 2Department of Biochemistry and Molecular Biology,University of Southern Denmark, 3Department of Cardiac, Thoracic and Vascular Surgery,Odense University Hospital

Chapters

  • 00:04Title
  • 01:02Imaging of the Artery Using TPEM
  • 02:47Diameter and Wall Thickness Measurement
  • 03:33Imaging the Collagen and Elastin Microarchitecture
  • 05:10Image Analysis Internal Elastic Lamina Branching Angles
  • 05:51Image Analysis – Collagen Fiber Straightness
  • 07:46Results: Measurement of the Microstructural Changes in Adventitial Collagen and the Internal Elastic Lamina in Response to Vessel Pressure
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述同时进行的机械测试和 3 d 成像的动脉壁的孤立, 活着的人的抵抗动脉, 斐济和 Ilastik 图像分析, 以量化的弹性蛋白和胶原的空间组织和体积密度。我们讨论了这些数据在动脉壁力学数学模型中的应用。

Tags

CollagenElastinMicroarchitectureResistance ArteriesLabel-free Fluorescence MicroscopyTwo-photon MicroscopyExtracellular MatrixVascular MechanicsArtery PressurizationSecond Harmonic GenerationAutofluorescenceFiji Image Analysis

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