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用无标签荧光显微镜评价活体、人体阻力动脉中胶原蛋白和弹性蛋白的压力依赖性 Microarchitectures
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
DOI:

09:58 min

April 09, 2018

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Chapters

  • 00:04Title
  • 01:02Imaging of the Artery Using TPEM
  • 02:47Diameter and Wall Thickness Measurement
  • 03:33Imaging the Collagen and Elastin Microarchitecture
  • 05:10Image Analysis Internal Elastic Lamina Branching Angles
  • 05:51Image Analysis – Collagen Fiber Straightness
  • 07:46Results: Measurement of the Microstructural Changes in Adventitial Collagen and the Internal Elastic Lamina in Response to Vessel Pressure
  • 08:48Conclusion

Summary

Automatic Translation

我们描述同时进行的机械测试和 3 d 成像的动脉壁的孤立, 活着的人的抵抗动脉, 斐济和 Ilastik 图像分析, 以量化的弹性蛋白和胶原的空间组织和体积密度。我们讨论了这些数据在动脉壁力学数学模型中的应用。

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