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Genetics
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基于纠错 DNA 和 RNA 测序的稀有事件检测方法
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
基于纠错 DNA 和 RNA 测序的稀有事件检测方法
DOI:
10.3791/57509-v
•
10:36 min
•
August 03, 2018
•
Wing H. Wong*
1,2
,
R. Spencer Tong*
1,2
,
Andrew L. Young
2
,
Todd E. Druley
2
1
Department of Pediatrics, Division of Hematology and Oncology
,
Washington University School of Medicine
,
2
Center for Genome Sciences and Systems Biology
,
Washington University School of Medicine
Chapters
00:04
Title
00:33
Incorporating i5 and i7 Adapters onto Error-corrected Sequencing Libraries
03:36
Quantifying the Libraries with QX200 Digital Droplet PCR (ddPCR)
06:14
Amplifying and Normalizing the Libraries for Sequencing
07:37
Results: Mutation-calling with Error-corrected Sequencing (ECS)
09:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
下一代测序 (0.5–2.0%) 是基因组特征的有力工具, 受平台高错误率的限制。我们描述了我们的错误纠正排序方法, 使我们能够避免的误差率和检测突变的变异等位基因分数罕见的0.0001。
Tags
Rare Event Detection
Error-corrected DNA Sequencing
Error-corrected RNA Sequencing
Clonal Single-nucleotide Variants
Small Indels
Unique Molecular Identifiers
Q5 Master Mix
PCR
Magnetic Bead Cleanup
Illumina Chemistry
Gene Panels
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