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用迈尔斯测定法评价皮肤血管 Hyperpermeability 诱导剂的含量
JoVE Journal
Biology
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JoVE Journal Biology
Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
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用迈尔斯测定法评价皮肤血管 Hyperpermeability 诱导剂的含量

DOI:
10.3791/57524-v

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08:43 min

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June 19, 2018

•

, ,
1UCL Institute of Ophthalmology,University College London

Chapters

  • 00:04Title
  • 01:01Intravenous Injection of Evans Blue Dye
  • 02:48Stimulation of Vascular Hyperpermeability
  • 04:27Quantification of the Accumulated Evans Blue within the Dermis
  • 06:22Results: Use of the Miles Assay to Detect Vascular Leakage Triggered by Intradermal Injection of VEGF into C57/BI6 Mice
  • 07:39Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个协议, 以测量的血管泄漏诱导的皮下渗透促进剂进入小鼠皮肤。该技术可用于确定分子促进或抑制血管渗漏的能力, 或研究调节血管通透性的分子机制。

Tags

Vascular HyperpermeabilityMiles AssayVascular LeakagePermeability Promoting AgentsVascular PermeabilityEdemaPyrilamine MaleateEvans Blue DyeIntraperitoneal InjectionVasodilationIntravenous InjectionVEGFPhosphate Buffered Saline

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