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从小鼠眼中激光捕捉高纯大梁网的基因表达分析
JoVE Journal
Biology
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JoVE Journal Biology
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
DOI:

13:47 min

June 03, 2018

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Chapters

  • 00:05Title
  • 01:00Frozen Section Preparation for Laser Microdissection
  • 04:25Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol
  • 06:14Laser Microdissection with UV Laser
  • 11:01Results: Evaluation of RNA Quality and Downstream RNA Analysis
  • 12:25Conclusion

Summary

Automatic Translation

在这里, 我们描述了一个可重现的激光捕获显微切割 (LCM) 的协议, 用于分离小梁网 (TM) 的下游 RNA 分析。分析 tm 基因表达变化的能力有助于了解 tm 相关眼部疾病的基本分子机制。

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