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从小鼠眼中激光捕捉高纯大梁网的基因表达分析
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Biology
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JoVE Journal
Biology
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠眼中激光捕捉高纯大梁网的基因表达分析
DOI:
10.3791/57576-v
•
13:47 min
•
June 03, 2018
•
Caleb Sutherland*
1
,
Yu Wang*
2
,
Robert V. Brown*
1
,
Julie Foley
,
Beth Mahler
,
Kyathanahalli S. Janardhan
3
,
Ramesh C. Kovi
4
,
Anton M. Jetten
1
Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research
,
National Institute of Environmental Health Sciences, NIH
,
2
Cellular and Molecular Pathology Branch, Division of the National Toxicology Program
,
National Institute of Environmental Health Sciences, NIH
,
3
Integrated Laboratory Systems Inc.
,
4
Experimental Pathology Laboratories Inc.
Chapters
00:05
Title
01:00
Frozen Section Preparation for Laser Microdissection
04:25
Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol
06:14
Laser Microdissection with UV Laser
11:01
Results: Evaluation of RNA Quality and Downstream RNA Analysis
12:25
Conclusion
Summary
Automatic Translation
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在这里, 我们描述了一个可重现的激光捕获显微切割 (LCM) 的协议, 用于分离小梁网 (TM) 的下游 RNA 分析。分析 tm 基因表达变化的能力有助于了解 tm 相关眼部疾病的基本分子机制。
Tags
Laser Microdissection
Trabecular Meshwork
Gene Expression Analysis
Ocular Diseases
RNA Isolation
Cryostat Sectioning
H&E Staining
Laser Capture Microscopy
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