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从人类获得变性 Tenocyte 的一种协议
JoVE Journal
Medicine
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JoVE Journal
Medicine
A Protocol to Acquire the Degenerative Tenocyte from Humans
Please note that all translations are automatically generated.
Click here for the English version.
从人类获得变性 Tenocyte 的一种协议
DOI:
10.3791/57634-v
•
09:25 min
•
June 09, 2018
•
Soo-Hong Han*
1
,
Hyung Kyung Kim*
2
,
Jong-Ho Ahn*
1
,
Dong Hyeon Lee
,
Minjung Baek
,
Geunhee Ye
,
Joong-Myung Lee
,
Kyunghoon Min
,
Chihoon Oh
,
Soonchul Lee
1
Department of Orthopaedic Surgery, CHA Bundang Medical Center
,
CHA University School of Medicine
,
2
Department of Pathology, Kyung Hee University Hospital at Gangdong
,
Kyung Hee University School of Medicine
,
3
Department of Physiology
,
CHA University School of Medicine
,
4
Department of Rehabilitation, CHA Bundang Medical Center
,
CHA University School of Medicine
Chapters
00:04
Title
01:01
Tendon Tissue Sample Extraction
02:39
Tenocyte Culture and Immunocytochemistry (ICC)
05:10
Hematoxylin and Eosin (H&E) and Immunohistochemical (IHC) Analysis
07:36
Results: Representative Characteristics of Degenerative Tenocytes
08:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
体外
应用退行性肌腱细胞是必要的, 当调查的效果, 新的治疗肌腱。然而, 大多数研究研究只使用动物模型或健康的 tenocyte。我们提出了以下的协议, 以隔离人类退行性肌腱细胞在手术期间。
Tags
Degenerative Tenocyte
Cell Culture
Collagenase Digestion
Tenocyte Isolation
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